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The regulated movement of proteins and lipids between the many cellular membranes in coated vesicular carriers is important for signaling, homeostasis, defining the interactions of cells with their surroundings, and in controlling the glycosylation and proteolytic processing of lumenal and transmembrane proteins. Clathrin-coated vesicles CCVs mediate many post-Golgi trafficking routes including internalization from the plasma membrane via clathrin-mediated endocytosis CME Traub, Transmembrane proteins are generally selected as cargo for incorporation into a CCV through the direct interaction of either widely used, short, linear sequence motifs or covalently attached ubiquitin chains with the membrane-proximal portion of a clathrin adaptor reviewed in Bonifacino and Traub, ; Traub, The appendages are connected to the core via long flexible linkers Zaremba and Keen, that contain clathrin-binding motifs. The AP2 complex therefore acts as a central hub for CCV formation, binding to clathrin, cargo molecules, accessory proteins, and membranes, and these interactions must be under strict spatiotemporal control. Top: overall composite view of the whole AP2 complex in its locked conformation.
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QUILT BINDING TUTORIAL — Farm & Folk

I think I will try your 2" binding technique soon I have a plastic container full of waiting binding packets in all colorways that I need to use first! Hugs, sao in Midlothian, VA. When you are joining the last strips of binding together after sewing on the quilt, make a pleat in your quilt and hold it with a safety pin. It won't pull on your binding when you are sewing it together. Jill O in Montana. Thanks Bonnie. I do my last connection a little different, but also cut it a little "short" so there are no puckers.
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This is my most favorite method and I use it to bind all my quilts. This tutorial picks up where my 9-patch quilt tutorial left off. First you will need to trim any extra batting and backing from around the perimeter of your quilt. I like to do this with my rotary cutter and the longest quilting ruler I have.
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